诺金
骨形态发生蛋白
骨形态发生蛋白2
细胞生物学
骨愈合
SMAD公司
间充质干细胞
骨形态发生蛋白7
脚手架
材料科学
信号转导
生物医学工程
化学
医学
生物
体外
解剖
生物化学
基因
作者
Jiabing Fan,Xiao Zhang,Cecı́lia Leal,Chung-Sung Lee,Lauren Kim,Danny Hadaya,Tara Aghaloo,Min Lee
出处
期刊:Biomaterials
[Elsevier]
日期:2023-11-01
卷期号:302: 122335-122335
标识
DOI:10.1016/j.biomaterials.2023.122335
摘要
The bone morphogenetic protein (BMP) signaling pathway plays a crucial role in bone development and regeneration. While BMP-2 is widely used as an alternative to autograft, its clinical application has raised concerns about adverse side effects and deteriorated bone quality. Therefore, there is a need to develop more sophisticated approaches to regulate BMP signaling and promote bone regeneration. Here, we present a novel complementary strategy that targets both BMP antagonist noggin and agonist Trb3 to enhance bone defect repair without the application of exogenous BMP-2. In vitro studies showed that overexpression of Trb3 with simultaneous noggin suppression significantly promotes osteogenic differentiation of mesenchymal stem cells. This was accompanied by increased BMP/Smad signaling. We also developed sterosome nanocarriers, a non-phospholipid liposomal system, to achieve non-viral mediated noggin suppression and Trb3 overexpression. The gene-loaded sterosomes were integrated onto an apatite-coated polymer scaffold for in vivo calvarial defect implantation, resulting in robust bone healing compared to BMP-2 treatments. Our work provides a promising alternative for high-quality bone formation by regulating expression of BMP agonists and antagonists.
科研通智能强力驱动
Strongly Powered by AbleSci AI