Toxicity of sulfhydryl attapulgite to Eisenia fetida

Sulfhydryl attapulgite is an effective material for soil remediation, but its potential toxicity to animals in soil is not fully understood. Using Eisenia fetida as the test organism, the avoidance experiments, acute toxicity and cumulative toxicity experiments were carried out. The avoidance rate of Eisenia fetida on ATP-SH is 0–60%. Eisenia fetida not only avoid, but also tend to the soil-added ATP-SH to a certain degree. In the acute toxicity test, with the increase of ATP-SH addition, the total protein (T-Protein) content gradually decreases, and the maximum rate of decline is 51.18%. The catalase (CAT) and superoxide dismutase (T-SOD) activities gradually increase, and the maximum increase rates are 73.56% and 51.73%, respectively. The malondialdehyde (MDA) activity significantly increase 1.8 times. In the cumulative toxicity experiment, with the increase of ATP-SH addition, the maximum increase rate of T-Protein content is 31.52%. The CAT and T-SOD activities decrease 64.38% and 43.60% at most, respectively, and MDA activity increase 0.9 times. Following exposure to the soil-added ATP-SH for 30 days, Eisenia fetida adapt to the environment through its own regulatory mechanism. ATP-SH has good application security in remediation of heavy metal-contaminated soil.