生物
拟南芥
生物钟
选择性拼接
昼夜节律
RNA剪接
细胞生物学
基因表达
拟南芥
转录组
MYB公司
基因
遗传学
基因表达调控
基因亚型
突变体
核糖核酸
神经科学
作者
Allan B. James,Chantal Sharples,Janet Laird,Emily May Armstrong,Wenbin Guo,Nikoleta A. Τzioutziou,Runxuan Zhang,John W. Brown,Hugh G. Nimmo,Matthew A. Jones
摘要
Cold stress is one of the major environmental factors that limit growth and yield of plants. However, it is still not fully understood how plants account for daily temperature fluctuations, nor how these temperature changes are integrated with other regulatory systems such as the circadian clock. We demonstrate that REVEILLE2 undergoes alternative splicing after chilling that increases accumulation of a transcript isoform encoding a MYB-like transcription factor. We explore the biological function of REVEILLE2 in Arabidopsis thaliana using a combination of molecular genetics, transcriptomics, and physiology. Disruption of REVEILLE2 alternative splicing alters regulatory gene expression, impairs circadian timing, and improves photosynthetic capacity. Changes in nuclear gene expression are particularly apparent in the initial hours following chilling, with chloroplast gene expression subsequently upregulated. The response of REVEILLE2 to chilling extends our understanding of plants immediate response to cooling. We propose that the circadian component REVEILLE2 restricts plants responses to nocturnal reductions in temperature, thereby enabling appropriate responses to daily environmental changes.
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