HOF-101-based dual-mode biosensor for photoelectrochemical/electrochemiluminescence detection and imaging of oxytetracycline

A unique hydrogen-bonded organic frameworks (HOF-101)-based photoelectrochemical (PEC) and electrochemiluminescence (ECL) dual-mode biosensor using polydopamine nanoparticles (PDAs) as quencher was constructed for ultrasensitive detection and imaging of oxytetracycline (OXY). In particular, HOF-101 was a superior ECL material and can be observed with the naked eye. Furthermore, it also had outstanding PEC signal, so HOF-101 was a new dual-signal material with excellent performance, thus it was explored to realize dual-mode detection. As the main component of natural melanin, PDAs not only had good biocompatibility, but also contained rich functional groups on the surface. Additionally, PDAs had excellent light absorption ability and poor conductivity, which made it the excellent photoquencher. In this work, PDAs were introduced on the surface of HOF-101 to quench its ECL and PEC signals by using the dual-aptamer sandwich method, achieving ultrasensitive detection of antibiotic OXY. Particularly for ECL detection, HOF-101 was firstly used to visually detect OXY. The detection range can reach 0.1 pM-100 nM, and the limit of detection (LOD) can reach 0.04 pM. This work showed a great contribution to the development of new ECL-PEC materials and ECL visualization analysis, which had outstanding application potential in the fields of food safety and biochemical analysis.