三甲胺
氧化三甲胺
检出限
单加氧酶
化学
吸光度
含黄素单加氧酶
基质(水族馆)
黄素组
色谱法
酶
有机化学
生物
细胞色素P450
生态学
作者
Shiwangi Maurya,Abhishek Kumar Singh,Gurunath Ramanathan
出处
期刊:Proteins
[Wiley]
日期:2023-09-29
卷期号:93 (1): 3-10
摘要
Trimethylamine monooxygenase (Tmm, EC-1.14.13.148) belongs to the family of flavin-containing monooxygenases that oxidize trimethylamine into trimethylamine-N-oxide (TMAO). Conventional methods for assaying Tmm are accurate over a narrow range of substrate/product concentrations. Here we report a TMAO-specific enzymatic assay for Tmm using polyallylamine hydrochloride (PAHCl)-capped MnO
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