上皮-间质转换
SMAD公司
癌症研究
波形蛋白
子宫内膜癌
转移
转化生长因子
庆大霉素保护试验
体内
医学
异甘草素
蛋白激酶B
信号转导
PI3K/AKT/mTOR通路
内科学
MAPK/ERK通路
细胞生物学
癌症
癌变
生物
药理学
免疫组织化学
生物技术
作者
Hsin Yuan Chen,Yi Fen Chiang,Jing Huang,Tsui-Chin Huang,Yin Hwa Shih,Kai-Lee Wang,Mohamed Ali,Yong Han Hong,Tzong‐Ming Shieh,Shih Min Hsia
出处
期刊:Cancers
[MDPI AG]
日期:2021-03-11
卷期号:13 (6): 1236-1236
被引量:18
标识
DOI:10.3390/cancers13061236
摘要
Endometrial cancer is a common gynecological cancer with a poor prognosis, mostly attributed to tumor metastasis. Epithelial–mesenchymal transition (EMT) can be mediated via transforming growth factor beta (TGF-β) signaling pathway, facilitating the ability of cancer cell invasion and migration. Isoliquiritigenin (ISL) is a flavonoid derived from licorice with reported antineoplastic activities. This study aims to investigate the anti-metastatic potential of ISL on endometrial cancer both in vitro and in vivo. First, human endometrial cancer cell lines (HEC-1A, Ishikawa, and RL95-2) were treated with ISL and then subjected to functional assays such as migration assay as well as molecular analyses including immunoblotting, immunofluorescence and RT-qPCR. In addition, HEC-1A-LUC cells were implanted into female nude mice and treated with ISL by intraperitoneal injection for four weeks. Results showed that ISL inhibited cell migration and reversed the effect of TGF-β on the expression of E-cadherin, N-cadherin, vimentin, α-SMA, p-Smad3, and TWIST1/2 In vitro. Interestingly, In vivo study revealed that ISL reduced peritoneal dissemination and serum level of TGF-β1, as well as decreased the expression levels of N-cadherin, p-Smad2/3, TWIST1/2, while increased E-cadherin. Overall, ISL reverses the EMT through targeting the TGF-β/Smad signaling pathway and features a potential therapeutic treatment for metastatic endometrial cancer.
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