A fluorogenic H2S donor activated by reactive oxygen species for real-time monitoring in cells and in vivo

Hydrogen sulfide (H2S) is an important gasotransmitter in biological system, and plays a crucial role in varied physiological and pathological processes. Exogenous H2S is widely employed as a positive control in H2S related biological study. Herein, we develop a reactive oxygen species (ROS) triggered donor HSD545 that delivers H2S and simultaneously generates a fluorophore to real-time monitoring the process of H2S release in vitro and in vivo. The donor exhibits low cytotoxicity and strong cytoprotection against ROS-induce oxidative stress.