纤维素酶
碎石
垃圾箱
植物凋落物
化学
木质素
微生物种群生物学
酶分析
有机质
食品科学
农学
植物
生物
酶
生态学
细菌
营养物
生物化学
遗传学
作者
Jian Wu,Qian Zhang,Dandan Zhang,Wei Jia,Ji Chen,Guihua Liu,Xiaoli Cheng
标识
DOI:10.1016/j.apsoil.2021.104269
摘要
Soil extracellular enzymes play a central role in mediating the decomposition of soil organic matter (SOM), and the activities of cellulase and ligninase therein quantify the preference of microbial carbon (C) utilization. However, the responses of cellulase and ligninase activities to plant detritus input change remain uncertain. Here, we investigated the activities of cellulase and ligninase after two years of detritus input manipulations (the detritus input and removal treatment (DIRT) include a control without litter manipulation, CK; double litter, DL; no litter, NL; no roots, NR; no litter and no roots, NRNL) in a coniferous forest in subtropical China. The litter removal treatments had negligible effect on cellulase activity, while the DL treatment significantly increased it by 55.7% compared to the CK treatment. The NL and NRNL treatments increased the activity of ligninase by 60.1% and 46.9%, respectively. However, the DL treatment did not significantly affect the activity of ligninase. Consequently, the ratio of ligninase to cellulase significantly increased under the litter removal treatments. Notably, the specific enzyme activity (the amount of enzyme produced per unit microbial biomass) increased under the litter removal treatment, but the DL treatment did not significantly affect it. The increased ratio of ligninase to cellulase under the litter removal treatments was primarily driven by the increased recalcitrance of substrates and higher proportion of fungal and gram-positive bacterial community. Moreover, the specific enzyme activity and ratio of ligninase to cellulase were positively correlated with microbial metabolic quotient (qCO2). Overall, our results provided an empirical evidence that microorganisms could shift substrate-using strategy by upregulating the production of ligninase with the reduction of plant detritus input. More importantly, the enhanced activity or proportion of ligninase resulted in higher qCO2, and thereby can accelerate soil C loss once plant detritus input is decreased caused by some global change drivers.
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