化学
胶体金
吸光度
纳米颗粒
细菌
悬挂(拓扑)
细菌细胞结构
细菌生长
肉眼
活力测定
致病菌
色谱法
纳米技术
生物化学
检出限
体外
生物
遗传学
数学
材料科学
纯数学
同伦
作者
Takamasa Kinoshita,Kengo Ishiki,Dung Quang Nguyen,Hiroshi Shiigi,Tsutomu Nagaoka
标识
DOI:10.1021/acs.analchem.7b05439
摘要
Real-time evaluation of bacterial viability is important for various purposes such as hygiene management, development of antibacterial agents, and effective utilization of bacterial resources. Here, we demonstrate a simple procedure for evaluating bacterial viability using gold nanoparticles (Au NPs). The color of bacterial suspensions containing Au NPs strongly depended on the bacterial viability. We found that the dispersion state of Au NPs affected the color of the suspension, based on the interaction of Au NPs with substances secreted by the bacteria. This color change was easily recognized with the naked eye, and viability was accurately determined by measuring the absorbance at a specific wavelength. This method was applicable to various bacterial species, regardless of whether they were Gram-positive or Gram-negative.
科研通智能强力驱动
Strongly Powered by AbleSci AI