拟南芥
叶绿体
细胞生物学
化学
鉴定(生物学)
蛋白质亚细胞定位预测
作者
Luo Jun,Ryoichi Saiki,Kei Tatsumi,Tsuyoshi Nakagawa,Makoto Kawamukai
出处
期刊:Plant and Cell Physiology
[Oxford University Press]
日期:2004-12-15
卷期号:45 (12): 1882-1888
被引量:58
摘要
;Two solanesyl diphosphate synthases, designated SPS1 and SPS2, which are responsible for the synthesis of the isoprenoid side chain of either plastoquinone or ubiquinone in Arabidopsis thaliana, were identified. Heterologous expression of either SPS1 or SPS2 allowed the generation of UQ-9 in a decaprenyl diphosphate synthase-defective strain of fission yeast and also in wild-type Escherichia coli. SPS1–GFP was found to localize in the ER while SPS2–GFP localized in the plastid of tobacco BY-2 cells. These two different subcellular localizations are thought to be the reflection of their roles in solanesyl diphosphate synthesis in two different parts: presumably SPS1 and SPS2 for the side chains of ubiquinone and plastoquinone, respectively.
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