Recognizing a Single Base in an Individual DNA Strand: A Step Toward DNA Sequencing in Nanopores

The capture of single strands of DNA inside the α-hemolysin (α-HL) transmembrane pore protein upon application of a positive potential leads to single α-HL⋅DNA pseudorotaxane species (see picture). By monitoring the characteristic decreases in the ion conductance of α-HL, a single adenine nucleotide can be identified at a specific location on a strand of DNA. Supporting information for this article is available on the WWW under http://www.wiley-vch.de/contents/jc_2002/2005/z462114_s.pdf or from the author. Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.