生物
病毒学
腱鞘炎
胚胎
抗体
拉伤
成纤维细胞
接种
减毒疫苗
免疫学
医学
毒力
细胞培养
解剖
外科
基因
细胞生物学
生物化学
遗传学
作者
L. van der Heide,M. Kalbac,Maryanne Brustolon
出处
期刊:Avian Diseases
[BioOne (American Association of Avian Pathologists)]
日期:1983-07-01
卷期号:27 (3): 698-698
被引量:49
摘要
A fully attenuated apathogenic reovirus vaccine was developed by 235 serial passages of S1133 strain avian reovirus in embryonating chicken eggs and 100 additional passages in chicken embryo fibroblast (CEF) cultures, 65 of which were cultured at 32 C. Chickens with and without maternal antibodies to avian reovirus were vaccinated subcutaneously at 1 day of age and challenged via footpad at 14 days of age. It appeared that the 40th, 66th, and 100th CEF passage levels were apathogenic at doses ranging from 10(2.5) to 10(6.8) TCID50/chick. No gross or microscopic lesions of tenosynovitis developed in vaccinated chicks. Vaccinated chicks were protected against challenge; unvaccinated control chickens were not.
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