酶
化学
过氧化氢酶
生物化学
生物催化
酒
生物物理学
组合化学
催化作用
生物
离子液体
作者
Yang Liu,Juanjuan Du,Ming Yan,Mo Yin Lau,Jay Hu,Hongwei Han,Otto O. Yang,Sheng Liang,Wei Wei,Hui Wang,Jianmin Li,Xinyuan Zhu,Linqi Shi,Wei Chen,Ji Cheng,Yunfeng Lu
标识
DOI:10.1038/nnano.2012.264
摘要
Organisms have sophisticated subcellular compartments containing enzymes that function in tandem. These confined compartments ensure effective chemical transformation and transport of molecules, and the elimination of toxic metabolic wastes1,2. Creating functional enzyme complexes that are confined in a similar way remains challenging. Here we show that two or more enzymes with complementary functions can be assembled and encapsulated within a thin polymer shell to form enzyme nanocomplexes. These nanocomplexes exhibit improved catalytic efficiency and enhanced stability when compared with free enzymes. Furthermore, the co-localized enzymes display complementary functions, whereby toxic intermediates generated by one enzyme can be promptly eliminated by another enzyme. We show that nanocomplexes containing alcohol oxidase and catalase could reduce blood alcohol levels in intoxicated mice, offering an alternative antidote and prophylactic for alcohol intoxication. Two or more enzymes encapsulated in a thin polymer shell can lower blood alcohol levels in intoxicated mice, offering a way to prevent liver injury arising from the overconsumption of alcohol.
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