福尔马赞
电子受体
化学
琥珀酸脱氢酶
氯化四氮唑
接受者
氯化物
酶
酶分析
脱氢酶
色谱法
生物化学
有机化学
心脏病学
物理
缺血
医学
凝聚态物理
作者
Petraki Munujos,Jaume Coll-Cantí,F González-Sastre,F J Gella
标识
DOI:10.1006/abio.1993.1360
摘要
A spectrophotometric assay method for determining succinate dehydrogenase activity is described in which iodonitrotetrazolium chloride is used as a final electron acceptor. The enzyme activity is determined by measuring the formation of formazan due to the tetrazolium salt reduction. The assay is continuous, rapid, simple, and sensitive, and may be used in the determination of enzyme activity either in tissue homogenates or as a marker of the mitochondrial fraction in cell fractionation procedures.
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