圆二色性
化学
胍
胰高血糖素
盐酸盐
甲醇
超离心机
溶解度
催化作用
药物化学
立体化学
有机化学
色谱法
生物化学
激素
标识
DOI:10.1016/0005-2795(72)90189-4
摘要
Glycineamide reacts quantitatively with the four carboxyl groups of glucagon in the presence of 1-ethyl-3-(3-dimethylaminopropylcarbodiimide) and 7 M guanidine hydrochloride. The product of this reaction has greatly enhanced solubility in water but still aggregates as shown by the concentration dependence of the circular dichroism and ultracentrifuge studies. The circular dichroism at pH 2.8 resembles that of glucagon. Blockage of the carboxyl groups by glycineamide or methanol results in the loss of the ability of the peptide to stimulate adenyl cyclase.
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