脆弱类杆菌
结直肠癌
胆汁酸
拟杆菌
胆酸
鹅去氧胆酸
癌症研究
生物
肿瘤进展
内科学
癌症
医学
微生物学
内分泌学
抗生素
遗传学
细菌
作者
Lulu Sun,Yi Zhang,Jie Cai,Bipin Rimal,Edson R. Rocha,James P. Coleman,Chenran Zhang,Robert G. Nichols,Yuhong Luo,Bora Kim,Yaozong Chen,Kristopher W. Krausz,Curtis C. Harris,Andrew D. Patterson,Zhipeng Zhang,Shogo Takahashi,Frank J. Gonzalez
标识
DOI:10.1038/s41467-023-36089-9
摘要
Abstract Bile salt hydrolase (BSH) in Bacteroides is considered a potential drug target for obesity-related metabolic diseases, but its involvement in colon tumorigenesis has not been explored. BSH-expressing Bacteroides is found at high abundance in the stools of colorectal cancer (CRC) patients with overweight and in the feces of a high-fat diet (HFD)-induced CRC mouse model. Colonization of B. fragilis 638R, a strain with low BSH activity, overexpressing a recombinant bsh gene from B. fragilis NCTC9343 strain, results in increased unconjugated bile acids in the colon and accelerated progression of CRC under HFD treatment. In the presence of high BSH activity, the resultant elevation of unconjugated deoxycholic acid and lithocholic acid activates the G-protein-coupled bile acid receptor, resulting in increased β-catenin-regulated chemokine (C-C motif) ligand 28 (CCL28) expression in colon tumors. Activation of the β-catenin/CCL28 axis leads to elevated intra-tumoral immunosuppressive CD25 + FOXP3 + T reg cells. Blockade of the β-catenin/CCL28 axis releases the immunosuppression to enhance the intra-tumoral anti-tumor response, which decreases CRC progression under HFD treatment. Pharmacological inhibition of BSH reduces HFD-accelerated CRC progression, coincident with suppression of the β-catenin/CCL28 pathway. These findings provide insights into the pro-carcinogenetic role of Bacteroides in obesity-related CRC progression and characterize BSH as a potential target for CRC prevention and treatment.
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