Enhanced detection of N-acetylneuraminic acid using a chemiluminescence enzyme-linked immunosorbent assay

. Spiked recovery experiments using edible bird's nest, milk, saliva, and urine confirmed the CLEIA's high stability, sensitivity, minimal matrix effects, and suitability for food quality control, safety, clinical, and biomedical applications. This advancement sets a new benchmark in analytical detection and application of Neu5Ac over traditional methods, promising to revolutionize food safety monitoring and clinical diagnostics.