An odorant receptor tuned to an attractive plant volatile vanillin in Spodoptera litura

斜纹夜蛾 吸引力 气味 嗅觉系统 香兰素 爪蟾 触角电图 嗅觉 昆虫 植物 有害生物分析 生物 幼虫 生物化学 生态学 神经科学 哲学 基因 语言学
作者
Zhiqiang Wei,Ji‐Xiang Wang,Jin‐Meng Guo,Xiaolong Liu,Qi Yan,Jin Zhang,Shuang-Lin Dong
出处
期刊:Pesticide Biochemistry and Physiology [Elsevier BV]
卷期号:196: 105619-105619 被引量:9
标识
DOI:10.1016/j.pestbp.2023.105619
摘要

The insect olfaction plays crucial roles in many important behaviors, in which ORs are key determinants for signal transduction and the olfactory specificity. Spodoptera litura is a typical polyphagous pest, possessing a large repertoire of ORs tuning to broad range of plant odorants. However, the specific functions of those ORs remain mostly unknown. In this study, we functionally characterized one S. litura OR (OR51) that was highly expressed in the adult antennae. First, by using Xenopus oocyte expression and two-electrode voltage clamp recording system (XOE-TEVC), OR51 was found to be strongly and specifically responsive to vanillin (a volatile of S. litura host plants) among 77 tested odorants. Second, electroantennogram (EAG) and Y-tube behavioral experiment showed that vanillin elicited significant EAG response and attraction behavior especially of female adults. This female attraction was further confirmed by the oviposition experiment, in which the soybean plants treated with vanillin were significantly preferred by females for egg-laying. Third, 3D structural modelling and molecular docking were conducted to explore the interaction between OR51 and vanillin, which showed a high affinity (-4.46 kcal/mol) and three residues (Gln163, Phe164 and Ala305) forming hydrogen bonds with vanillin, supporting the specific binding of OR51 to vanillin. In addition, OR51 and its homologs from other seven noctuid species shared high amino acid identities (78-97%) and the same three hydrogen bond forming residues, suggesting a conserved function of the OR in these insects. Taken together, our study provides some new insights into the olfactory mechanisms of host plant finding and suggests potential applications of vanillin in S. litura control.
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