A direct, real-time, size-resolved analytical strategy to follow drug loading and release from biocompatible gold nanoparticles

The tailored strategy employed in our investigation provided detailed, real-time, in situ analysis, making it a powerful tool for designing and optimizing drug delivery systems, providing insight into both loading and release mechanisms, assessing nanoparticle stability, and tolerating saline media. These results suggest that AF4-DAD-MALS is a more reliable and insightful technique for studying the stability, loading efficiency, and release dynamics of nanoparticle-based drug delivery systems.