Methionine affects the freeze‐drying resistance of Lactiplantibacillus plantarum LIP‐1 by improving its antioxidant capacity

BACKGROUND: Lactic acid bacteria is an essential industrial strain, and improving its freeze-drying survival rate is the key challenge to ensuring the activity and stability of bacterial powder. Although medium optimization has been shown to strengthen strain freeze-drying tolerance, the mechanism by which amino acids repair freeze-drying damage in lactic acid bacteria remains unclear. This study investigated the effects of methionine on the freeze-drying survival rate of Lactiplantibacillus plantarum LIP-1 and explored the underlying protective mechanisms. RESULTS: The study demonstrates that supplementing the medium with 0.06 g/L methionine significantly improved the freeze-drying survival rate of Lactiplantibacillus plantarum LIP-1 (P < 0.05). Further analysis revealed that the strain significantly reduced intracellular reactive oxygen species levels through metabolizing methionine (P < 0.05), decreased the oxidation degree of unsaturated fatty acids in the cell membrane, and reduced cell membrane damage, thereby strengthening the freeze-drying resistance of the strain. CONCLUSION: Methionine can enhance the freeze-drying resistance of Lactiplantibacillus plantarum LIP-1 by enhancing antioxidant capacity and maintaining the stability of the subcellular structure. This study provides a specific reference value for improving the freeze-drying survival rate of lactic acid bacteria by modifying the medium conditions. © 2025 Society of Chemical Industry.