细胞外基质
组织工程
诱导多能干细胞
收缩(语法)
Ⅰ型胶原
细胞生物学
哺乳动物心脏
生物医学工程
解剖
材料科学
化学
生物
生物化学
医学
病理
内科学
胚胎干细胞
基因
作者
Hideaki Tani,Eiji Kobayashi,Shinomi Yagi,Keisuke Tanaka,Kotaro Kameda-Haga,Shinsuke Shibata,Nobuko Moritoki,Kaworu Takatsuna,Taijun Moriwaki,Otoya Sekine,Tomohiko Umei,Yuika Morita,Y Soma,Yoshikazu Kishino,Hideaki Kanazawa,Jun Fujita,Shunji Hattori,Keiichi Fukuda,Shugo Tohyama
出处
期刊:Biomaterials
[Elsevier]
日期:2023-08-01
卷期号:299: 122174-122174
被引量:4
标识
DOI:10.1016/j.biomaterials.2023.122174
摘要
Although the extracellular matrix (ECM) plays essential roles in heart tissue engineering, the optimal ECM components for heart tissue organization have not previously been elucidated. Here, we focused on the main ECM component, fibrillar collagen, and analyzed the effects of collagens on heart tissue engineering, by comparing the use of porcine heart-derived collagen and other organ-derived collagens in generating engineered heart tissue (EHT). We demonstrate that heart-derived collagen induces better contraction and relaxation of human induced pluripotent stem cell-derived EHT (hiPSC-EHT) and that hiPSC-EHT with heart-derived collagen exhibit more mature profiles than those with collagens from other organs. Further, we found that collagen fibril formation and gel stiffness influence the contraction, relaxation, and maturation of hiPSC-EHT, suggesting the importance of collagen types III and type V, which are relatively abundant in the heart. Thus, we demonstrate the effectiveness of organ-specific collagens in tissue engineering and drug discovery.
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