生物
斑马鱼
消音器
再生(生物学)
细胞生物学
计算生物学
遗传学
基因
机械工程
工程类
入口
作者
Kazunori Ando,Jianhong Ou,John Thompson,John Welsby,Sushant Bangru,Jingwen Shen,Xiaolin Wei,Yarui Diao,Kenneth D. Poss
标识
DOI:10.1016/j.devcel.2024.01.004
摘要
Regeneration involves gene expression changes explained in part by context-dependent recruitment of transcriptional activators to distal enhancers. Silencers that engage repressive transcriptional complexes are less studied than enhancers and more technically challenging to validate, but they potentially have profound biological importance for regeneration. Here, we identified candidate silencers through a screening process that examined the ability of DNA sequences to limit injury-induced gene expression in larval zebrafish after fin amputation. A short sequence (s1) on chromosome 5 near several genes that reduce expression during adult fin regeneration could suppress promoter activity in stable transgenic lines and diminish nearby gene expression in knockin lines. High-resolution analysis of chromatin organization identified physical associations of s1 with gene promoters occurring preferentially during fin regeneration, and genomic deletion of s1 elevated the expression of these genes after fin amputation. Our study provides methods to identify “tissue regeneration silencer elements” (TRSEs) with the potential to reduce unnecessary or deleterious gene expression during regeneration.
科研通智能强力驱动
Strongly Powered by AbleSci AI