Ventral Tegmental Area Glutamatergic Neurons Facilitated Emergence From Isoflurane Anesthesia Involves Excitation of Lateral Septum γ-Aminobutyric Acid-ergic Neurons in Mice

谷氨酸的 被盖腹侧区 光遗传学 异氟醚 神经科学 医学 荷包牡丹碱 麻醉 加巴能 谷氨酸受体 内科学 生物 多巴胺能 多巴胺 γ-氨基丁酸受体 受体 抑制性突触后电位
作者
Simin Zhang,Xinxin Zhang,Huiming Li,Dan Wang,Sa Wang,Yuhao Wang,Guangchao Zhao,Hailong Dong,Jiannan Li
出处
期刊:Anesthesia & Analgesia [Lippincott Williams & Wilkins]
被引量:5
标识
DOI:10.1213/ane.0000000000006739
摘要

BACKGROUND: Ventral tegmental area (VTA) glutamatergic neurons promote wakefulness in the sleep-wake cycle; however, their roles and neural circuit mechanisms during isoflurane (ISO) anesthesia remain unclear. METHODS: Fiber photometry and in vivo electrophysiology were used to observe the changes in neuronal or terminal activity during ISO anesthesia and arousal processes. Optogenetic and anesthesia behaviors were used to investigate the effects of VTA glutamatergic neurons and their projections to the lateral septum (LS) during ISO anesthesia and arousal. Anterograde and retrograde tracings were performed to identify the connections between VTA glutamatergic neurons and the LS. RESULTS: Population activity and firing rates of VTA glutamatergic neurons decreased during ISO anesthesia (ISO: 95% confidence interval [CI], 0.83–2.06 Spikes.s –1 vs wake: 95% CI, 3.53–7.83 Spikes.s –1 ; P =.0001; n = 34 from 4 mice). Optogenetic activation of VTA glutamatergic neurons reduced the burst-suppression ratio in electroencephalography (laser: 95% CI, 13.09%–28.76% vs pre: 95% CI, 52.85%–71.59%; P =.0009; n = 6) and facilitated emergence (ChR2: 95% CI, 343.3–388.0 seconds vs mCherry: 95% CI, 447.6–509.8 seconds; P < .0001; n = 11/12) from ISO anesthesia. VTA glutamatergic neurons monosynaptically innervated LS γ-aminobutyric acid (GABA)-ergic neurons. The activity of VTA glutamatergic terminals in the LS decreased during ISO anesthesia, and optogenetic activation of the VTA glutamatergic terminals in the LS facilitated emergence from ISO anesthesia. Furthermore, optogenetic activation of VTA glutamatergic terminals increased the firing rates of LS γ-aminobutyric acid-ergic (GABAergic) neurons (laser: 95% CI, 0.85–4.03 Spikes.s –1 vs pre: 95% CI, 0.24–0.78 Spikes.s –1 ; P =.008; n = 23 from 4 mice) during ISO anesthesia. CONCLUSIONS: VTA glutamatergic neurons facilitated emergence from ISO anesthesia involving excitation of LS GABAergic neurons.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
缓慢的如波完成签到,获得积分20
刚刚
1秒前
1秒前
SYLH应助songge采纳,获得50
1秒前
烟花应助ksen采纳,获得10
1秒前
RUHUAN发布了新的文献求助10
2秒前
抽烟不完成签到 ,获得积分10
3秒前
ldzjiao完成签到 ,获得积分10
4秒前
科研通AI5应助smile采纳,获得10
4秒前
隐形曼青应助echo采纳,获得10
6秒前
Amy完成签到 ,获得积分10
10秒前
12秒前
Taro完成签到 ,获得积分10
15秒前
111发布了新的文献求助10
16秒前
Muriel完成签到,获得积分10
17秒前
爆米花应助缓慢的如波采纳,获得50
20秒前
雪白的如天完成签到 ,获得积分10
21秒前
科研通AI5应助不知名采纳,获得10
22秒前
qingxinhuo完成签到 ,获得积分10
23秒前
suzy-123完成签到 ,获得积分10
24秒前
26秒前
26秒前
tianliyan完成签到 ,获得积分10
27秒前
科研小迷糊完成签到,获得积分10
28秒前
29秒前
wqm发布了新的文献求助10
29秒前
长岛冰茶W发布了新的文献求助10
30秒前
33秒前
科研通AI5应助科研通管家采纳,获得10
33秒前
彭于晏应助科研通管家采纳,获得10
33秒前
赘婿应助科研通管家采纳,获得10
33秒前
33秒前
33秒前
所所应助科研通管家采纳,获得10
33秒前
科研通AI2S应助科研通管家采纳,获得10
33秒前
科研通AI5应助科研通管家采纳,获得10
33秒前
33秒前
佰斯特威应助南风采纳,获得10
34秒前
z_king_d_23发布了新的文献求助10
35秒前
35秒前
高分求助中
【此为提示信息,请勿应助】请按要求发布求助,避免被关 20000
Production Logging: Theoretical and Interpretive Elements 3000
CRC Handbook of Chemistry and Physics 104th edition 1000
Izeltabart tapatansine - AdisInsight 600
Introduction to Comparative Public Administration Administrative Systems and Reforms in Europe, Third Edition 3rd edition 500
Distinct Aggregation Behaviors and Rheological Responses of Two Terminally Functionalized Polyisoprenes with Different Quadruple Hydrogen Bonding Motifs 450
Individualized positive end-expiratory pressure in laparoscopic surgery: a randomized controlled trial 400
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 物理 生物化学 纳米技术 计算机科学 化学工程 内科学 复合材料 物理化学 电极 遗传学 量子力学 基因 冶金 催化作用
热门帖子
关注 科研通微信公众号,转发送积分 3761727
求助须知:如何正确求助?哪些是违规求助? 3305495
关于积分的说明 10134394
捐赠科研通 3019564
什么是DOI,文献DOI怎么找? 1658199
邀请新用户注册赠送积分活动 791974
科研通“疑难数据库(出版商)”最低求助积分说明 754751