Effect of fibronectin on proliferation, adhesion and migration in cultured primary open-angle glaucoma trabecular meshwork cells

Objective To study the relationship between fibronectin and primary open-angle glaucoma (POAG) pathogenesis by investigating the effect of fibronectin on the proliferation, adhesion and migration of human trabecular meshwork cells (HTC) in POAG patients. Methods Using HTC that were successfully established from the albuginea oculi in the trabeculectomy of patients, HTC were cultivated for primary culture and subculture. The third passage cells were incubated with different doses of fibronectin (5, 10, 20, 40, 100 μg/ml) cultivated with DMEM/F12. No fibronectin was used in the control group. In order to see the effect of different doses of fibronectin on cell proliferation, adhesion and migration, a Cell Counting Kit-8 and Transwell Kit were used after 24 hours of cultivation. The number of living cells was calculated to detect the optical density value and the data was used to calculate the results, with SPSS 17.0 statistics software. The normal distribution of data between the 2 groups was analyzed with a One-Way ANOVA. SNK test was used to compare the difference between groups. Results POAG trabecular meshwork cells were identified and confirmed with immunocytochemistry and electron microscopy. After 24 hours, a serum -free culture was incubated with different doses of fibronectin (5, 10, 20, 40, 100 μg/ml) cultivated with DMEM/F12. Different doses of fibronectin (5, 10, 20, 40, 100 μg/ml) significantly promote the proliferation of POAG trabecular meshwork cells. However, the proliferation of POAG trabecular meshwork cells with lower doses of fibronectin (5, 10 μg/ml) promote a relatively slow proliferation. There was an upward trend with an increase after the dosage of 10 μg/ml, and proliferation of trabecular meshwork cells reached a peak with higher doses of fibronectin (40 μg/ml). High doses of fibronectin (100 μg/ml) still promote the proliferation of cells, but the proliferation is relatively slow. The difference was significant compared to the control group (P<0.01). Fibronectin (5, 10, 20, 40, 100 μg/ml) promotes adhesion and migration of POAG trabecular meshwork cells, that showed an upward trend. The difference was significant compared to the control group (P<0.05). Conclusion Based on this data, it is possible that fibronectin affects intraocular pressure by affecting the proliferation, adhesion and migration of trabecular meshwork cells POAG patients that control outflow in the trabecular meshwork. Key words: Primary open-angle glaucoma;  Trabecular meshwork cell,human;  Fibronectin;  Cell proliferation;  Cell adhesion;  Cell migration