生物修复
微生物联合体
石油
生物降解
环境科学
环境修复
微生物种群生物学
生化工程
生物技术
生物刺激
化学
生物
细菌
污染
工程类
微生物
生态学
有机化学
遗传学
作者
Jie Cui,Ling Huang,Weiwei Wang,Ping Xu,Giulio Zanaroli,Hongzhi Tang
标识
DOI:10.1016/j.ibiod.2020.104964
摘要
Petroleum pollution is a global concern because it causes serious environmental and human health problems. Microbial communities play important roles in petroleum biodegradation. In this study, a synthetic bacterial consortium for efficiently degrading ability was constructed on the basis of a minimal value algorithm running on MATLAB. Functional microbial diversity-based screening of three petroleum-degrading strains, Achromobacter sp. P3, Sphingobium sp. P10 and Rhizobium sp. P14, enabled the initial synthesis of the bacterial consortium in combination with a previously used bacterial consortium PDM. When 27.0% of P3, 21.0% of P10, 28.0% of P14 and 24.0% of consortium PDM were inoculated to a petroleum medium, 78.9% of petroleum was degraded under the optimal conditions. The synthetic bacterial consortium has 34.8% higher petroleum degradation than the original bacterial community PDM. Total 40% of the petroleum in the contaminated soil was removed after 20 days of soil bioremediation using the reconstructed consortium. This is an attempt to remediate petroleum-contaminated pollution with synthetic biology via reconstructing a synthetic bacterial community, providing a new idea for the remediation of other pollution problems.
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