生物
转录组
神经干细胞
基因敲除
大脑皮层
细胞生物学
祖细胞
长非编码RNA
祖细胞
转录因子
皮质激素生成
神经发生
胚胎干细胞
基因表达
神经科学
基因
干细胞
遗传学
核糖核酸
作者
Kuan Tian,Yuzhuo Wang,Junbao Wang,Wei Li,Wenchen Shen,Yamu Li,Zhiyuan Luo,Ying Liu,Yan Zhou
标识
DOI:10.1007/s12264-020-00607-2
摘要
Long non-coding RNAs (lncRNAs) regulate transcription to control development and homeostasis in a variety of tissues and organs. However, their roles in the development of the cerebral cortex have not been well elucidated. Here, a bioinformatics pipeline was applied to delineate the dynamic expression and potential cis-regulating effects of mouse lncRNAs using transcriptome data from 8 embryonic time points and sub-regions of the developing cerebral cortex. We further characterized a sense lncRNA, SenZfp536, which is transcribed downstream of and partially overlaps with the protein-coding gene Zfp536. Both SenZfp536 and Zfp536 were predominantly expressed in the proliferative zone of the developing cortex. Zfp536 was cis-regulated by SenZfp536, which facilitates looping between the promoter of Zfp536 and the genomic region that transcribes SenZfp536. Surprisingly, knocking down or activating the expression of SenZfp536 increased or compromised the proliferation of cortical neural progenitor cells (NPCs), respectively. Finally, overexpressing Zfp536 in cortical NPCs reversed the enhanced proliferation of cortical NPCs caused by SenZfp536 knockdown. The study deepens our understanding of how lncRNAs regulate the propagation of cortical NPCs through cis-regulatory mechanisms.
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