转录组
核糖核酸
细胞外小泡
计算生物学
化学
胞外囊泡
小RNA
核酸
细胞
微泡
细胞生物学
小泡
生物
生物化学
细胞外
基因表达
基因
膜
作者
María Galli de Amorim,Emmanuel Dias-Neto,Diana Noronha Nunes
出处
期刊:Methods in Enzymology
日期:2020-01-01
卷期号:: 141-154
标识
DOI:10.1016/bs.mie.2020.08.001
摘要
Extracellular vesicles (EVs) have been recognized as relevant players in cell-cell communication. To fully explore their potential as carriers of biological information in clinical settings, protocols capable of dealing with minute amounts of proteins, lipids, and nucleic acids present in their cargo are a requirement. Here we delve into a protocol to decipher the total transcriptome of EVs, from undetectable amounts of EVs-derived RNA from clinical samples.
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