前病毒
数字聚合酶链反应
牛白血病病毒
病毒学
生物
实时聚合酶链反应
血清学
猫白血病病毒
群体特异性抗原
白血病
套式聚合酶链反应
病毒
Deltaretrovirus病毒
病毒载量
聚合酶链反应
分子生物学
基因
抗体
免疫学
遗传学
基因组
病毒性疾病
作者
Laureana De Brun,Bruno Cosme,Marcos Petersen,Irene Alvarez,Aurea Folgueras-Flatschart,Roberto Flatschart,Carlos Javier Panei,Rodrigo Puentes
标识
DOI:10.1177/10406387221085581
摘要
Droplet digital PCR (ddPCR) is a highly sensitive tool developed for the detection and quantification of short-sequence variants-a tool that offers unparalleled precision enabling measurement of smaller-fold changes. We describe here the use of ddPCR for the detection of Bovine leukemia virus (BLV) DNA provirus. Serum samples and whole blood from experimentally infected sheep and naturally infected cattle were analyzed through ddPCR to detect the BLV gp51 gene, and then compared with serologic and molecular tests. The ddPCR assay was significantly more accurate and sensitive than AGID, ELISA, nested PCR, and quantitative PCR. The limit of detection of ddPCR was 3.3 copies/µL, detecting positive experimentally infected sheep beginning at 6 d post-infection. The ddPCR methodology offers a promising tool for evaluating the BLV proviral load, particularly for the detection of low viral loads.
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