The impaired endothelial function is associated with number of disease states,including cardiovascular disease and its risk factors.Although no golden standard for the evaluation of endothelial dysfunction,the measurement of flow-mediated dilation in the brachial artery assessed by Doppler ultrasonography shows the most promise for clinical application.The aim of this article is reviewing the investigation advance and application value in the evaluation of endothelial function by ultrasound technique.