HPLC determination on related substances of cefazedone sodium

Objective:To develop an HPLC gradient elution method for the determination on the related substances of cefazedone sodium.Method:RP-HPLC was adopted.The stationary phase was C18.The mobile phase was 0.02 mol·L-1 a mmonium dihydrogen phosphate buffer(the pH value adjusted by 0.1 mol·L-1 sodium hydroxide solution to pH 5.0)-acetonitrile.The HPLC gradient elution was used.The flow rate was 1 mL·min-1.The detection wavelength was 278 nm.Results:The impurities of cefazedone sodium were isolated well.The LOD was 0.015 μg·mL-1.The RSD was 0.62%.Conclusion:This method is convient,quick and has good repeatability.This method can be adopted to detect the related substances of cefazedone sodium one at a time.