Design,Synthesis and Characterization of Novel Fluorescein-based Calcium Fluorescent Probe Fluo-Cl

The role of intracellular calcium ions is of high interest in the fields of cellular biology.Calcium plays a critical role in regulating numerous physiological cellular phenomena.There is a close relation between the change in the consent ration of free calcium and the function of the cell,signal transmission and the cell injury or death.So it is very important to determine the consentration and the distribution of the intracellular free calcium.Fluorescent indicators have been widely used for imaging calcium.They are highly sensitive and offer imaging by fluorescent microscopy in an easier and less cell damaging way than other methods.In this paper we describe the design,synthesis,characterization and spectral characteristic of novel fluorescein-derived calcium fluorescent probes.The fluorescent spectrum and the calcium titration curve of the novel compound Fluo-Cl were reported for the first time.The synthesis of fluorescein-based calcium fluorescent probe Fluo-Cl was researched.The calcium titration curve of Fluo-Cl shows that after chelating with calcium the fluorescent relative intensity has been enhanced greatly.