Construction of cDNA Library of Hylarana latouchii Skin Tissue with Cloning and Sequence Analysis of the cDNA Encoding Antimicrobial Peptides Brevinin-2LTs

A full length cDNA library of Hylarana latouchii skin tissue was constructed with the SMART technique.This cDNA library could be used to screen encoding gene of antimicrobial peptides and other functional genes.The titer of the cDNA library was 9.7×106 cfu/mL,and the recombination rate of it was 91%.Inserted fragments ranged was 0.3-1.5 kb.The titer of the amplified library was 1.9×109 cfu/mL.Successfully constructed cDNA library was a full length library with high quality.Two different cDNA sequences encoding antimicrobial peptide precursors named brevinin-2LT1 and brevinin-2LT2 were cloned by PCR from this cDNA library.All of the two antimicrobial peptides attributed to brevinin-2 family.