免疫沉淀
盘基网柄菌
蛋白质-蛋白质相互作用
细胞内
细胞生物学
信号转导
化学
生物
质谱法
蛋白质组学
鉴定(生物学)
计算生物学
生物化学
基因
植物
色谱法
出处
期刊:Methods in molecular biology
日期:2016-01-01
卷期号:: 131-139
被引量:3
标识
DOI:10.1007/978-1-4939-3480-5_10
摘要
Protein-protein interactions play central roles in intercellular and intracellular signal transduction. Impairment of protein-protein interactions causes many diseases such as cancer, cardiomyopathies, diabetes, microbial infections, and genetic and neurodegenerative disorders. Immunoprecipitation is a technique in which a target protein of interest bound by an antibody is used to pull down the protein complex out of cell lysates, which can be identified by mass spectrometry. Here, we describe the protocol to immunoprecipitate and identify the components of the protein complexes of ElmoE in Dictyostelium discoideum cells.
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