Exosome-transmitted microRNA-323a-3p participated in the occurrence of Hirschsprung’s disease

BACKGROUND: Hirschsprung's disease (HSCR) is caused by defective enteric neural crest cell (ENCC) migration. Exosome-transmitted microRNAs are implicated in HSCR pathogenesis, but mechanisms remain unclear. METHODS: Plasma exosomes and colon tissues were collected from HSCR patients and controls. We assessed the effects of exosomal miR-323a-3p on the proliferation and migration of immortalized ENCC-derived neural cell line (iENC) in vitro using CCK-8, EdU and Transwell assays, and its impact on ENCC migration in vivo using a zebrafish model. RESULTS: = 0.0043). Exosomal miR-323a-3p was taken up by iENCs and suppressed their proliferation and migration. TET2 was identified as a potential miR-323a-3p target. TET2 was downregulated in HSCR aganglionic tissues, and its knockdown inhibited iENC proliferation and migration. In the zebrafish model, exosomal miR-323a-3p impaired distal ENCC colonization. CONCLUSION: Exosomal miR-323a-3p is upregulated in HSCR and associated with impaired ENCC-derived cell function, potentially via TET2. These findings suggest exosome-transmitted microRNA-323a-3p participated in the occurrence of Hirschsprung's disease and exhibit promising potential as a prospective diagnostic biomarker.