The cryopreservation of plant germplasm by vitrification

Cryopreservation of plant germplasm by vitrification technique has been appreciated extensively to date. Vitrification method consists of 5 steps mainly: loading, dehydration by protective solution of vitrification, cooling, warming and washing (namely unloading). There are hundreds of species of plants being cryopreserved by this method in the world up to now, but mainly of species of higher plants. However, few successful studies on the cryopreservation of algae germplasm by vitrification technique have been reported so far. Applying vitrification technique to cryopreservation of some species of algae may have significant potentia1.