显微镜
显微镜
光学
分辨率(逻辑)
材料科学
高分辨率
双光子激发显微术
单细胞分析
激光器
薄层荧光显微镜
图像分辨率
超分辨显微术
物理
生物
计算机科学
扫描共焦电子显微镜
荧光
细胞
人工智能
遥感
地质学
遗传学
作者
Rémi Galland,Gianluca Grenci,Ajay Aravind,Virgile Viasnoff,Vincent Studer,Jean‐Baptiste Sibarita
出处
期刊:Nature Methods
[Springer Nature]
日期:2015-05-11
卷期号:12 (7): 641-644
被引量:180
摘要
Single-objective selective-plane illumination microscopy (soSPIM) is achieved with micromirrored cavities combined with a laser beam-steering unit installed on a standard inverted microscope. The illumination and detection are done through the same objective. soSPIM can be used with standard sample preparations and features high background rejection and efficient photon collection, allowing for 3D single-molecule-based super-resolution imaging of whole cells or cell aggregates. Using larger mirrors enabled us to broaden the capabilities of our system to image Drosophila embryos.
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