生物过程
下游加工
单克隆抗体
瓶颈
色谱法
工艺工程
化学
生产力
消耗品
计算机科学
化学工程
工程类
生物
抗体
嵌入式系统
宏观经济学
物理化学
经济
免疫学
作者
Mario Grünberg,Kim K. Kuchemüller,Katrin Töppner,Ricarda A. Busse
出处
期刊:Membranes
[MDPI AG]
日期:2022-06-30
卷期号:12 (7): 677-677
被引量:4
标识
DOI:10.3390/membranes12070677
摘要
The recombinant monoclonal antibody capture step represents the current bottleneck in downstream processing. Protein A resins are diffusion-limited chromatography materials which require low flow rates to achieve a binding capacity above 30 g L-1 with the result of low productivity. Here, we present a novel chromatography membrane combining superior binding capacities with high flow rates for high productivity while achieving comparable product quality as state-of-the-art protein A resins. Further, we demonstrate full scalability of this convecdiff technology with experimental data demonstrating suitability for bioprocessing at different scales. This technology results in more than 10-fold higher productivity compared to Protein A resins, which is maintained during scale up. We demonstrate the influence of residence times, feed titers and the cleaning regime on productivity and indicate optimal utilization of the convecdiff membrane based on feed titer availability. The underlying high productivity and short cycle times of this material enable the purification of monoclonal antibodies with 10-times less chromatography material used per batch and utilization of the membrane within one batch. Provided in disposable consumables, this novel technology will remove column handling in bioprocesses and resin re-use over multiple batches.
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