注意事项
尿酸
比色法
校准曲线
生物医学工程
检出限
普鲁士蓝
计算机科学
医学
化学
色谱法
病理
内科学
电极
物理化学
电化学
作者
Kexin Fan,Jiayang Zeng,Chenyu Yang,Gonglei Wang,Kai Lian,Xiuhong Zhou,Yaping Deng,Guozhen Liu
出处
期刊:ACS Sensors
[American Chemical Society]
日期:2022-07-12
卷期号:7 (7): 2049-2057
被引量:27
标识
DOI:10.1021/acssensors.2c00854
摘要
Uric acid (UA) is an important biomarker for many diseases. A sensitive point-of-care (POC) testing platform is designed for the digital quantification of salivary UA based on a colorimetric reaction on an easy-to-build smartphone-assisted microfluidic paper-based analytical device (SμPAD). UA levels are quantified according to the color intensity of Prussian blue on the SμPAD with the aid of a MATLAB code or a smartphone APP. A color correction method is specifically applied to exclude the light effect. Together with the engineering design of SμPADs, the background calibration function with the APP increases the UA sensitivity by 100-fold to reach 0.1 ppm with a linear range of 0.1–200 ppm. The assay time is less than 10 min. SμPADs demonstrate a correlation of 0.97 with a commercial UA kit for the detection of salivary UA in clinical samples. SμPADs provide a sensitive, fast, affordable, and reliable tool for the noninvasive POC quantification of salivary UA for early diagnosis of abnormal UA level-associated health conditions.
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