白色念珠菌
微生物学
生物膜
类胡萝卜素
抗菌剂
白色体
生物
克鲁斯假丝酵母
肉汤微量稀释
抗菌肽
细菌
最小抑制浓度
遗传学
作者
Michele Scarsini,Linda Tomasinsig,Alessandra Arzese,Francesca D’Este,Débora Oro,Barbara Skerlavaj
出处
期刊:Peptides
[Elsevier]
日期:2015-09-01
卷期号:71: 211-221
被引量:47
标识
DOI:10.1016/j.peptides.2015.07.023
摘要
Vulvovaginal candidiasis (VVC) is a frequent gynecological condition caused by Candida albicans and a few non-albicans Candida spp. It has a significant impact on the quality of life of the affected women also due to a considerable incidence of recurrent infections that are difficult to treat. The formation of fungal biofilm may contribute to the problematic management of recurrent VVC due to the intrinsic resistance of sessile cells to the currently available antifungals. Thus, alternative approaches for the prevention and control of biofilm-related infections are urgently needed. In this regard, the cationic antimicrobial peptides (AMPs) of the innate immunity are potential candidates for the development of novel antimicrobials as many of them display activity against biofilm formed by various microbial species. In the present study, we investigated the in vitro antifungal activities of the cathelicidin peptides LL-37 and BMAP-28 against pathogenic Candida spp. also including C. albicans, isolated from vaginal infections, and against C. albicans SC5314 as a reference strain. The antimicrobial activity was evaluated against planktonic and biofilm-grown Candida cells by using microdilution susceptibility and XTT [2,3-bis(2-methoxy-4-nitro-5-sulfo-phenyl)-2H-tetrazolium-5-carboxanilide] reduction assays and, in the case of established biofilms, also by CFU enumeration and fluorescence microscopy. BMAP-28 was effective against planktonically grown yeasts in standard medium (MIC range, 2–32 μM), and against isolates of C. albicans and Candida krusei in synthetic vaginal simulated fluid (MIC range 8–32 μM, depending on the pH of the medium). Established 48-h old biofilms formed by C. albicans SC5314 and C. albicans and C. krusei isolates were 70–90% inhibited within 24 h incubation with 16 μM BMAP-28. As shown by propidium dye uptake and CFU enumeration, BMAP-28 at 32 μM killed sessile C. albicans SC5314 by membrane permeabilization with a faster killing kinetics compared to 32 μM miconazole (80–85% reduced biofilm viability in 90 min vs 48 h). In addition, BMAP-28 at 16 μM prevented Candida biofilm formation on polystyrene and medical grade silicone surfaces by causing a >90% reduction in the viability of planktonic cells in 30 min. LL-37 was overall less effective than BMAP-28 against planktonic Candida spp. (MIC range 4–≥64 μM), and was ineffective against established Candida biofilms. However, LL-37 at 64 μM prevented Candida biofilm development by inhibiting cell adhesion to polystyrene and silicone surfaces. Finally, Candida adhesion was strongly inhibited when silicone was pre-coated with a layer of BMAP-28 or LL-37, encouraging further studies for the development of peptide-based antimicrobial coatings.
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