斯卡查德图
绘图(图形)
配体(生物化学)
同种类的
饱和(图论)
化学
摩尔浓度
非线性系统
数学
结合位点
受体
统计
物理
组合数学
生物化学
有机化学
量子力学
作者
H. J. G. Hollemans,Rogier M. Bertina
出处
期刊:Clinical Chemistry
[Oxford University Press]
日期:1975-11-01
卷期号:21 (12): 1769-1773
被引量:33
标识
DOI:10.1093/clinchem/21.12.1769
摘要
In saturation analysis the Scatchard plot is a generally accepted method for calculation of the affinity constant, K, and the molar concentration, q, of the binder. However, in a system where the K's for the labeled and unlabeled ligand are unequal, a nonlinear plot can be obtained from which incorrect values for K and q may be calculated. This paper mathematically explains how the plot may deviate and under which conditions there will be a maximum in the curve. When the binding sites are homogeneous, the coordinates of this maximum can be used to calculate K and q. A general mathematical expression is derived on the basis of which a linear curve can be constructed for calculation of q and K, which is valid even when affinity for the labeled and unlabeled ligand is not identical.
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