移植
神经干细胞
活力测定
祖细胞
干细胞
透明质酸
诱导多能干细胞
神经母细胞
材料科学
基质(化学分析)
细胞生物学
细胞
细胞分化
生物医学工程
医学
癌症研究
胚胎干细胞
生物
外科
神经发生
解剖
生物化学
复合材料
基因
作者
Jonathan Lam,William E. Lowry,S. Thomas Carmichael,Tatiana Segura
标识
DOI:10.1002/adfm.201401483
摘要
Stroke is the leading cause of adult disability with ≈80% being ischemic. Stem cell transplantation has been shown to improve functional recovery. However, the overall survival and differentiation of these cells is still low. The infarct cavity is an ideal location for transplantation as it is directly adjacent to the highly plastic peri‐infarct region. Direct transplantation of cells near the infarct cavity has resulted in low cell viability. Here, neural progenitor cells derived from induce pluripotent stem cells (iPS‐NPC) are delivered to the infarct cavity of stroked mice encapsulated in a hyaluronic acid hydrogel matrix to protect the cells. To improve the overall viability of transplanted cells, each step of the transplantation process is optimized. Hydrogel mechanics and cell injection parameters are investigated to determine their effects on the inflammatory response of the brain and cell viability, respectively. Using parameters that balanced the desire to keep surgery invasiveness minimal and cell viability high, iPS‐NPCs are transplanted to the stroke cavity of mice encapsulated in buffer or the hydrogel. While the hydrogel does not promote stem cell survival one week post‐transplantation, it does promote differentiation of the neural progenitor cells to neuroblasts.
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