转录因子ⅡH
解旋酶
核苷酸切除修复
色素性干皮病
Cockayne综合征
生物
分子生物学
DNA修复
遗传学
转录因子
一般转录因子
转录因子ⅡA
基因
发起人
基因表达
核糖核酸
作者
Laurent Schaeffer,Richard Roy,Sandrine Humbert,Vincent Moncollin,Wim Vermeulen,Jan H.J. Hoeijmakers,Pierre Chambon,Jean‐Marc Egly
出处
期刊:Science
[American Association for the Advancement of Science (AAAS)]
日期:1993-04-02
卷期号:260 (5104): 58-63
被引量:765
标识
DOI:10.1126/science.8465201
摘要
The human BTF2 basic transcription factor (also called TFIIH), which is similar to the delta factor in rat and factor b in yeast, is required for class II gene transcription. A strand displacement assay was used to show that highly purified preparation of BTF2 had an adenosine triphosphate-dependent DNA helicase activity, in addition to the previously characterized carboxyl-terminal domain kinase activity. Amino acid sequence analysis of the tryptic digest generated from the 89-kilodalton subunit of BTF2 indicated that this polypeptide corresponded to the ERCC-3 gene product, a presumed helicase implicated in the human DNA excision repair disorders xeroderma pigmentosum and Cockayne's syndrome. These findings suggest that transcription and nucleotide excision repair may share common factors and hence may be considered to be functionally related.
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