化学
生物等效性
托吡酯
药代动力学
色谱法
选择性反应监测
高效液相色谱法
串联质谱法
药理学
质谱法
医学
精神科
癫痫
作者
Jin Hee Park,Yoo-Sin Park,Minho Lee,Si-Youn Rhim,Jaechul Song,Soojin Lee,Jung Mogg Kim,Leslie M. Shaw,Ju‐Seop Kang
摘要
Abstract A rapid, simple and validated liquid chromatography coupled to tandem mass spectrometric method (LC‐MS/MS) for topiramate analysis in human plasma has been applied to pharmacokinetic and bioequivalence studies in 24 healthy male Korean volunteers. The procedure involves a simple liquid extraction of topiramate and prednisone (internal standard) with acetonitrile and separation by HPLC equipped with a Capcell Pak C 18 column using acetonitrile–0.1% triethylamine (80:20, v/v) as a mobile phase. Detection was carried out on an API 2000 MS system by multiple reactions monitoring mode. The ionization was optimized using ESI(−) and selectivity was achieved by MS/MS analysis, m / z 338.0 → 77.5 and m / z 357.1 → 327.2 for topiramate and prednisone, respectively. The method had a total run time of 2.5 min and showed good linearity over a working range of 20–5000 ng/mL in human plasma with a lower limit of quantification of 20 ng/mL. No metabolic compounds were found to interfere with the analysis. The inter‐day and intra‐day accuracy were in the ranges of 99.24–116.63 and 93.45–108.68%, respectively, and inter‐day and intra‐day precisions were below 6.24 and 5.25%, respectively. This method was successfully applied for pharmacokinetic and bioequivalence studies by analysis of blood samples taken up to 96 h after an oral administration of 100 mg of topiramate in 24 healthy Korean volunteers. Copyright © 2008 John Wiley & Sons, Ltd.
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