免疫抑制
环磷酰胺
化学
壳聚糖酶
药理学
免疫刺激剂
脾脏
脂多糖
脾细胞
卡拉胶
抗菌剂
免疫学
体外
免疫系统
酶
生物化学
生物
化疗
医学
内科学
有机化学
作者
Yuxia Mei,Hongxia Chen,Jun Zhang,Zhang Xuedan,Yunxiang Liang
标识
DOI:10.1016/j.ijbiomac.2013.09.038
摘要
Chitooligosaccharides (COS) display a variety of important biological activities, including antimicrobial, antitumor, anti-inflammatory, and immunoenhancing. In the present study, a COS sample (degree of polymerization 4–11, analyzed by FTIR and MALDI-TOF-MS) prepared by hydrolysis with a recombinant chitosanase was orally administered to mice for evaluation of its effect on cyclophosphamide (Cy)-induced immunosuppression. Thymus and spleen indices, delayed-type hypersensitivity (DTH) reaction, macrophage phagocytosis, and certain enzyme activities were significantly higher in mice treated with COS + Cy than in mice treated with Cy alone. ELISA experiments showed that COS treatment enhanced production of the cytokines IL-2, IL-12, and IFN-γ but decreased production of IL-10 in sera of Cy-treated mice. The well-defined COS product studied displayed strong immunoenhancing activity and a protective effect against Cy-induced immunosuppression. COS-derived products should be further investigated for possible immunostimulatory applications in the food and pharmaceutical industries.
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