碘化丙啶
细胞凋亡
枸杞
活力测定
MTT法
细胞生长
细胞毒性T细胞
细胞毒性
生物
细胞培养
癌细胞
传统医学
细胞
细胞周期
化学
分子生物学
癌症研究
药理学
癌症
生物化学
程序性细胞死亡
医学
体外
病理
替代医学
遗传学
作者
Anna Wawruszak,Arkadiusz Czerwonka,Karolina Okła,Wojciech Rzeski
标识
DOI:10.1080/14786419.2015.1101691
摘要
The anticancer activity of ethanol extract isolated from Goji berry (EEGB) on T47D human breast cancer cell line has been reported. Cell viability and cell proliferation were examined with the use of BrdU, MTT and NR methods. Induction of apoptosis was assessed by propidium iodide and Hoechst 33342 staining. Expression of genes involved in cell proliferation, apoptosis, cell cycle control and regulation of transcription was estimated using Western blotting analysis. EEGB inhibited the proliferation of breast cancer cells in a time-, and dose-dependent manner. The study confirmed the lack of EEGB cytotoxic activity to normal human skin fibroblasts. Western blot analysis demonstrated an increase in pro-apoptotic and a decrease in anti-apoptotic proteins’ expression in cells treated with the extract. Anticancer activity and lack of toxicity against normal cells indicate a chemopreventive potential of Goji berries in breast cancer treatment.
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