普鲁士蓝
生物传感器
胆固醇氧化酶
抗坏血酸
硅酸
化学
检出限
过氧化氢
核化学
电极
基质(化学分析)
无机化学
胆固醇
色谱法
有机化学
生物化学
电化学
食品科学
物理化学
作者
Jianping Li,Peng Tian,Yuqiang Peng
标识
DOI:10.1002/elan.200390124
摘要
Abstract A cholesterol biosensors fabricated by immobilization of cholesterol oxidase (ChOx) in a layer of silicic sol‐gel matrix on the top of a Prussian Blue‐modified glassy carbon electrode was prepared. It is based on the detection of hydrogen peroxide produced by ChOx at −0.05 V. The half‐lifetime of the biosensor is about 35 days. Cholesterol can be determined in the concentration range of 1×10 −6 −8×10 −5 mol/L with a detection limit of 1.2×10 −7 mol/L. Normal interfering compounds, such as ascorbic acid and uric acid do not affect the determination. The high sensitivity and outstanding selectivity are attributed to the Prussian Blue film modified on the sensor.
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