[Study on the biological assay of Herba Leonuri(I)--Establishment of standard uterus models and optimizing uterus environment conditions].

OBJECTIVE: To study the contracting uterus effect of Herba Leonuri, establish the standard uterus models, and optimize the environment conditions which could influence Herba Leonuri actions on uterus. METHODS: By comparing the contracting uterus effect of Herba Leonuri in mice, rats and rabbits, the standard uterus models was established. The optimum extra conditions of uterus were selected by orthogonal design methods. RESULTS: The isolated rat uterus pre-oestrus was selected for the standard uterus, the optimum uterus environment conditions as follows: the pre-burden of uterus was 1.0 g, the temperature of uterus nutrition solution is 32 degrees C, the assay of CaCl2 and NaHCO3 per 1000 ml nutrition solution was respectively 0.06 g and 0.25 g. CONCLUSION: Under the selected conditions, the contracting uterus effect of Herba Leonuri were more obvious and the errors of bioassay were less smaller.