Analysis of immunophenotype in 176 cases with acute leukemia by flow cytometry

Objective:To explore the acute leukemia immunophenotype and its diagnosis value.Methods: Three-colors monoclonal antibodies were used to mark the marrows of acute leukemia patients and CD45/SSC gating in flow cytometry was used for immunofluorescence.Results: The 176 cases of acute leukemia immunophenotype were classified into 3 subgroups: The undifferentiated phenotype(1 case) account for 0.7%,simple phenotype(101 cases) 57.3%,variant phenotype(74 cases) 42.0%.The variant phenotype,the scoring systems proposed by EGIL were adopted to classify,it's possible to identify Ly+-AML,My+-ALL,MAL.Conclusion: With the immunophenotype,every acute leukemia case can be analyzed,especially for those with mixed leukemia and cross-lineage expression leukemia.