The Isolation and Identification of the Porcine Pseudorabies Virus

The brain tissue of piglet was obtained and the 217 bp long fragment in gp50 gene was amplified by the polymerase chain reaction(PCR).Therefore,the piglet was confirmed as porcine pseudorabies virus infection.Subsequently,the porcine pseudorabies virus was isolated on BHK-21 cells after 5 generations.The cells displayed typical cell lesions and were identified as porcine pseudorabies virus by PCR.The virus infectivety of the pseudorabies virus was 108.68 TCID50/0.1 mL by Reed-Muench.Finally,the rabbits were inoculated with 107.0 TCID50/mL virus.On 48 h the injection sites showed typical itching,skin damage and other symptoms.The rabbits were dead after 72 h by the inoculation of pseudorabies virus.Consequently,the results indicated that the isolated pseudorabies virus was highly pathogenicity to susceptible animals.It would be the research foundation of the virus epidemiology,pathogenesis,vaccine and diagnositic.