The key point domain knowledge system is a type of knowledge base system with complex structure and functions.The function and performance of knowledge depend on the designing of knowledge base structure and searching technique,which are studied based on the ontology and domain knowledge system structure.The knowledge base structure is designed according to the objective data model theory,and realized the matching of knowledge component with the Elisa Bertino matching algorithm.The technique are used to develop the knowledge base of cigarette industry,and are verified in the knowledge management prototype system.